Screening, lipA Gene Amplification and Molecular Identification of Lipolytic Fungi from Contaminated Soils

Lipase is a glycerol ester hydrolase that hydrolyzes esters of triglycerides. This study aimed to isolate, screen and characterize lipolytic fungi with lipA gene from soil environments. Fungi [molds yeasts] were isolated contaminated samples randomly collected Palm oil mills, Abattoirs Automobile servicing workshops in Eleme Obio Akpor local governments Rivers State, Nigeria using standard microbiological techniques. Qualitative screening assay was carried out on the fungal isolates through gel diffusion Tributryin, Tween 80 Olive respectively. Isolates maximum zones clearance selected screened for hydrolysis triacylglyceride [lipA gene] amplification identified by Internal Transcribed Spacer characterization.
 A total 96 obtained, showed 57 strains which 21 palm 17 abattoirs 19 automobile Seven their genomic DNA as Candida orthopsilosis PMS3 [Accession No: OL546803], Fusarium proliferatum PMS4 OL546804], Saccharomyces cerevisiae PMS8 OL546805], Penicillium paxilli ABS8 OL546799], Pichia kudriavzevii ENS1 OL546800] Aspergillus niger ENS3 OL546801] Trichoderma viride ENS5 OL546802].
 are potential producers biotechnologically important lipase diverse industrial applications.